One-step versus two-step culture of mouse preimplantation embryos

One-step versus two-step culture of mouse preimplantation embryos.

BACKGROUND A comparison has been made of the development of mouse zygotes in either one-step or two-step culture systems. METHODS Embryo culture, blastocyst cell counts and embryo transfer were done. RESULTS No significant differences were observed in the proportions of blastocysts, rates of hatching, numbers of cells in the inner cell mass (ICM) and trophectoderm (TE) that developed in pro...

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Cost-effectivity analysis of one-step versus two-step screening for gestational diabetes.

OBJECTIVE Early diagnosis of gestational diabetes mellitus (GDM) is important for both maternal and fetal health. The literature has varying recommendations about one-step and two-step tests for GDM screening and diagnosis. The present study aimed to investigate the difference in the cost and duration of hospital stay of a one-step procedure compared to a two-step procedure, which is routinely ...

Complete preimplantation development in culture of parthenogenetic mouse embryos.

The present experiments were undertaken to determine whether, in parthenogenesis, heterozygous embryos develop better than homozygous embryos. Such experiments may provide an approach to elucidating whether fertilized embryos develop better than parthenogenetic ones because of heterozygosity, or if the sperm provides another contribution necessary for complete embryonic development. The parthen...

One-step and two-step bait fishing: Evaluation of results

The evaluation of the SILAC AP-MS experiments required a threshold for the SILAC ratio to define when an identified protein should be considered a contaminant or putative interaction partner. Other studies apply a statistical measure such as the Grubbs outlier test [1] or the z -test [2] to measure whether the deviation of a SILAC ratio is significant. These tests presuppose that the parameter ...